Streptavidin; Phycoerythrin Conjugate

Application

Enzyme Immunoassay (see comments)r>Flow Cytometry (1-3 µg per 1 x 10⁶ cells in 100 µl)

General description

Streptavidin conjugated to R-phycoerythrin. Specific for biotin, biotinylated proteins, or biotinylated antibodies.r>

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Lindmo, T., et al. 1990. J. Immunol. Methods126, 183.Starling, J.J., et al. 1988. Cancer Res.48, 6211.

The phycobiliprotein used in making this conjugate had a M.W. of 240,000. Molar extinction coefficient at 565 nm: 1.9 x 10⁶. Excitation Max: 500 nm emission max: 575 nm.

Packaging

1 ml in Plastic ampoule

Please refer to vial label for lot-specific concentration.

Physical form

In 150 mM NaCl, 50 mM sodium phosphate, 1% BSA, 0.1% glycerol, pH 7.6. Ammonium sulfate free.

Reconstitution

Do not dilute the entire vial at one time. Dilute only as needed in 50 mM sodium phosphate buffer, pH 7.2, 2-5 mg/ml of BSA or other physiological buffer.

Warning

Toxicity: Standard Handling (A)